This research will help determine the benefits, limitations, and accuracy of next-generation DNA sequencing in comparison to traditional taxonomic methods. Macroinvertebrate samples collected from wetlands in Folsom, CA, that were previously identified by a taxonomist, will be analyzed by next-generation sequencing (NGS). DNA will be extracted from both the isopropanol supernatant, used as the fixative and storage media for the samples, and from pooled and macerated macroinvertebrate tissue.The results of this study will help determine how next-generation sequencing can be used to benefit Reclamation projects that require environmental monitoring in response to construction activities, invasive species, habitat restoration, and water treatment.
